polyvalent human immunoglobulin (liquid) (Clairyg)

Management of Refractory Eosinophilic Fasciitis Using Biologic Therapies, Janus Kinase Inhibitors, and Intravenous Immunoglobulin: A Systematic Review.

Mitwalli Mohammed M, Waked Jihad Abou Ali JAA, Sood Siddhartha S, Abduelmula Abrahim A et al.

Validation and Quantification of Methamphetamine in Empty Puparia of Lucilia sericata (Diptera: Calliphoridae) Using Liquid-Liquid Extraction and Gas Chromatography-Mass Spectrometry.

Parkhideh Seyedeh Zahra SZ, Forouzesh Mehdi M, Akhgari Maryam M, Akbarzadeh Kamran K et al.

Entomotoxicology provides an alternative matrix for detecting drugs in decomposed human remains when conventional specimens are unavailable. This study aimed to develop and validate a quantitative method for detecting methamphetamine (MA) in different developmental stages of the blowfly Lucilia sericata. Immature stages of L. sericata (larvae, pupae, empty puparia) were reared on chicken liver spiked with MA at concentrations of 45, 90 and 180 ng/mg. Samples underwent liquid-liquid extraction, derivatization via acetylation, and analysis using gas chromatography-mass spectrometry (GC-MS). The method was validated according to international guidelines, evaluating limit of detection (LOD), limit of quantification (LOQ), linearity, precision, accuracy and repeatability. The method showed strong linearity across the studied concentration range (R2= 0.9817-0.9934). The limit of detection and LOQ were 15 ng/mg and 45 ng/mg, respectively, with a relative standard deviation (RSD)≤ 20% at the LOQ level. Intra- and inter-day precision showed RSD values< 10%, and accuracy ranged from 98.55% to 100.73%. Methamphetamine was detected in all immature stages and empty puparia. The highest observed concentrations were detected in third-instar larvae and pupae, whereas no drug residues were detected in adult flies. Methamphetamine retention in empty puparia supports their potential forensic relevance for retrospective drug detection. This validated method demonstrated acceptable sensitivity, precision, accuracy and repeatability for quantitative entomotoxicological analysis. The detection of methamphetamine across insect developmental stages suggests the suitability of this approach as a supplementary tool for forensic investigations involving decomposed remains.

Dual-Stimulus-Responsive Liquid Marbles with Programmable Motion on Liquid and Hydrogel Surfaces.

Goktas Cansu C, Aydogan Nihal N

Liquid marbles have attracted a significant amount of attention because of their dynamic programmable nature. In the present study, we propose the conceptualization and implementation of a novel dual-stimulus-responsive liquid marble (LM) platform actuated by near-infrared (NIR) light and magnetic fields and capable of exhibiting controllable locomotion behavior in response to changes in interfacial tension. The integration of external stimuli within a single liquid marble system has been achieved by means of two different micro- and nanoscale functional coating particles and a magnetically responsive core liquid. The liquid marble shell formed through the utilization of hydrophobic polypyrrole particles (SPP), which exhibit high photothermal energy in conjunction with hydrophobic magnetic nanoparticles (F-MNP), which provide magnetic responsiveness. In addition, ferrofluid (FF) has the capacity to respond to an applied magnetic field and is utilized as a core material. The resulting liquid marbles exhibited controllable locomotion under NIR irradiation, magnetic actuation, and combined stimuli through the utilization of near-infrared light-induced Marangoni flows and magnetic field-driven forces. In the presence of external stimuli, these systems generate asymmetric interfacial stress gradients at the liquid-air interface, resulting in the observed controllable locomotion. A systematic investigation was conducted to explore the impact of the particle type, magnetic field, and NIR exposure on the movement behavior of liquid marbles on water and polyacrylamide (PAM) hydrogel surfaces. This work demonstrates the potential of dual-stimulus-responsive liquid marbles as a programmable smart actuation system.

De Novo Proliferative Glomerulonephritis With Monoclonal Immunoglobulin Deposits (PGNMID) in a Renal Transplant Recipient.

Murugesan Ram Prabahar RP, Sivanandam Sathiyan S, Jayam Jayanivash J, Kurian Anila A AA

Proliferative glomerulonephritis with monoclonal immunoglobulin deposits (PGNMID) represents a distinct glomerular pathology, classified under monoclonal gammopathy of renal significance (MGRS). In renal transplant, PGNMID usually develops as a recurrent disease, but can rarely arise de novo. Recurrence is relatively common, typically appearing within five to six months post-transplant, and is linked to poor graft outcomes. De novo PGNMID is exceedingly rare, with few reported in the literature. It generally presents in the late post-transplant period, with a more indolent clinical course and a variable response to immunotherapy. This case report is of a 50-year-old patient who had diabetic nephropathy as his native kidney disease. This report documents a unique instance of de novo PGNMID, occurring three years post-transplantation with persistent allograft dysfunction. The transplant kidney biopsy showed mesangial hypercellularity with immunoglobulin (Ig)G and kappa light chain deposition by immunofluorescence; however, electron microscopy was non-contributory due to the absence of viable glomeruli. Despite extensive evaluation, we could not identify any clone contributing to the MGRS in the bone marrow, nor could we identify any other evidence of lymphoproliferative disease on positron emission tomography-computed tomography (PET-CT). We managed the patient with empirical clone-directed therapy against a likely B-cell clone using Rituximab. During rituximab therapy, the patient developed E. coli urosepsis, which was managed successfully. At the last follow-up, graft function remained stable without progression, although the duration of follow-up is limited.