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collagenase (Plaquase / Nucleolysin / lipolysin)

✓ Approved

Johnson & Johnson Services, Inc. · 治疗药物

什么是 collagenase？

collagenase 是一种治疗药物，由Johnson & Johnson Services, Inc.研发。该药已获批，用于治疗相关适应症，给药途径：Injectable (Others)、Topical。

药物档案

商品名Plaquase, Nucleolysin, lipolysin
公司Johnson & Johnson Services, Inc.
给药途径Injectable (Others), Topical
状态Approved
来源： ClinicalTrials.gov, Johnson & Johnson Services, Inc.

治疗适应症

collagenase 针对 10 个适应症，涉及 7 个治疗领域。

治疗领域疾病/病症分期
Skin and subcutaneous tissue disordersDecubitus ulcer✓ Approved
Injury, poisoning and procedural complicationsThermal burn✓ Approved
Skin and subcutaneous tissue disordersDiabetic foot✓ Approved
Musculoskeletal and connective tissue disordersDupuytren's contracture✓ Approved
Eye disordersGlaucoma✓ Approved

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相关研究文献

PubMedProtoplasma2026-10-26

Morphological, anatomical, and phytochemical characterization of Sisymbrium altissimum L.: chemical composition, biological activities, and isolation of major constituents.

Tekman Enes E, Aydın Bilge B, Yuca Hafize H, Özbek Hilal H et al.

This study comprehensively evaluated the phytochemical composition and multidimensional biological activities of Sisymbrium altissimum L., a wild edible species belonging to the Brassicaceae family. Methanolic extracts obtained from aerial parts, flowers, fruits, and roots were successively fractionated using solvents of increasing polarity, and essential and fatty oils were characterized by GC-MS and GC-FID analyses. The essential oil composition showed marked organ-dependent variation, with methallyl cyanide dominating fruit (91.9%) and flower (75.9%) oils, while phenylethyl isothiocyanate (48.7%) was the major constituent in roots. Further phytochemical investigation of the biologically active root hexane extract led to the isolation of β-sitosterol, lauric acid, and β-sitosterol-3-O-glucoside. Biological activity assays revealed strong α-glucosidase inhibition, particularly in the root hexane extract (87.92%), exceeding the reference drug acarbose (68.49%), whereas α-amylase inhibition was moderate, with the root ethyl acetate fraction exhibiting the highest activity (40.84%). Cholinesterase inhibition assays demonstrated low to moderate acetylcholinesterase and butyrylcholinesterase inhibitory activities depending on the plant part and extract fraction. Hyaluronidase inhibition was notably high, reaching up to 98.70%, whereas moderate collagenase (27.56%) and elastase (23.98%) inhibition and low tyrosinase activity were observed. Antioxidant activity was prominent in ethyl acetate and n-butanol fractions, with ABTS radical scavenging values approaching those of standard antioxidants. Total phenolic and flavonoid contents correlated with antioxidant capacity, although structural factors also influenced activity. Importantly, all tested extracts and cold-pressed oil exhibited no mutagenic or genotoxic effects in the Ames/Salmonella assay. Overall, these findings provide a comprehensive characterization of S. altissimum and highlight its potential as a source of biologically active natural products.

PMID 42928286
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PubMed3 Biotech2026-10-25

Development of cosmeceutical nanoemulsion formulation containing peptide acting on neurotransmitters and evaluation of its efficacy.

Gok Bahar B, Budama-Kilinc Yasemin Y, Kecel-Gunduz Serda S

External factors such as oxidative stress and ultraviolet (UV) radiation increase reactive oxygen species (ROS) and cause the skin to age faster. Recently, peptides have been frequently preferred in nanoformulation development to prevent these effects. This study aimed to develop a nanoemulsion formulation of Syn-Ake peptide that reduces wrinkles by affecting neurotransmitters and to evaluate its efficacy by in silico and in vitro methods. In this context, the anti-aging activity of Syn-Ake peptide was evaluated by molecular docking and MD simulation. Then, nanoemulsion formulations of the peptide were produced using ultrasonic homogenization method and stability and characterization studies were conducted. The in vitro safety of the formulation was evaluated by genotoxicity and cytotoxicity studies. The cell regenerative effect of the formulation was evaluated by scratch test. Additionally, the protective effect of its formulation against H2O2-UV-induced skin damage was evaluated at both the cellular and DNA levels. Finally, the antioxidant activity and the collagenase inhibitory activity of the formulation was evaluated. Our findings showed that the peptide strongly interacted with p-38 MAPK and PI3K-AKT signalling pathways, as well as stromelysins and gelatinases. The most stable formulation was determined among the formulations developed with different production procedures. The formulation was determined to be non-genotoxic, cytocompatible and to have cell regenerative properties. It was also evaluated that the formulation was protective against H2O2-UV-based damage, showed antioxidant activity and collagenase inhibitory activity. The formulation of Syn-Ake peptide in nanoemulsion was evaluated to have promising potential as an anti-aging product candidate with a high efficacy and safety profile. The online version contains supplementary material available at https://doi.org/10.1007/s13205-026-05090-6.

PMID 42927054
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PubMedTranslational stroke research2026-10-25

Pharmacological Inhibition of Hexokinase 2 Impairs Hematoma Clearance and Exacerbates Brain Injury After Intracerebral Hemorrhage in Mice.

Dai Jinyu J, Xie Yuxin Y, Yuan Ye Y, Zhang Zhongyi Z et al.

Erythrocyte-derived metabolites released from the hematoma contribute substantially to secondary brain injury after intracerebral hemorrhage (ICH), and enhancing endogenous hematoma clearance is a promising therapeutic strategy. Hexokinase 2 (HK2), a key glycolytic enzyme predominantly expressed in microglia, regulates microglial function, but its role in hematoma resolution after ICH remains unclear. We therefore investigated whether HK2 governs microglia/macrophage-mediated erythrophagocytosis and thereby influences hematoma clearance and neurological recovery after ICH. ICH was induced in male C57BL/6 mice by striatal collagenase injection, and HK2 was selectively inhibited with 3-bromopyruvate (3-BP). Neurobehavioral outcomes, hematoma volume, and hemoglobin content were quantified, and mechanisms were examined in hemoglobin- or erythrocyte-stimulated BV2 microglia and RAW 264.7 macrophages using pharmacological inhibition, glucose-6-phosphate (G-6-P) rescue, and lentiviral HK2 overexpression. After ICH, HK2 was localized predominantly to peri-hematomal microglia/macrophages, and nuclear HK2 increased progressively, peaking at 72 h. HK2 inhibition exacerbated neurological deficits, reduced M2-like (Arg-1⁺) microglia/macrophages, and delayed hematoma clearance without affecting initial hematoma formation. In vitro, G-6-P restored ATP levels suppressed by 3-BP but only partially rescued phagocytosis, indicating a non-glycolytic mechanism: HK2 upregulated peroxisome proliferator-activated receptor γ (PPARγ) and CD36, and pharmacological blockade of PPARγ abolished HK2-enhanced erythrophagocytosis. These findings identify HK2 as a contributor to endogenous hematoma clearance that promotes microglia/macrophage erythrophagocytosis at least in part through PPARγ/CD36 signaling, and support further genetic and cell-specific studies before HK2 is advanced as a therapeutic target for ICH.

PMID 42925448
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PubMedTrends in endocrinology and metabolism: TEM2026-10-23

The overlooked macrophages in adipose tissue.

Wu Huaizhu H, Ballantyne Christie M CM

Adipose tissue macrophages (ATMs) are heterogeneous, with distinct roles in metabolism. Prior studies mainly used collagenase-based stromal vascular fraction isolation to study ATM heterogeneity. Hu et al. employed multiple approaches, including a revised collagenase-based protocol, to investigate 'adhesion-related macrophages', substantially advancing our understanding of this often-overlooked ATM subpopulation.

PMID 42919745
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PubMedJournal of proteome research2026-10-23

Toxicological Exploration of Collagenase and Benzonase on Rat Precision-Cut Liver Slices.

Stam Janine J, Olinga Peter P, Menke Aswin A, Annaert Pieter P et al.

Precision-cut liver slices (PCLSs) are an ex vivo liver model that allows isolation of extracellular vesicles (EVs), which are nano-sized particles involved in intercellular communication. However, EV recovery from PCLSs is hindered by adhesion to the extracellular matrix and macromolecules such as extracellular DNA. Enzymatic treatments, such as collagenase (COL) and the endonuclease benzonase (BEN), can aid their release, but potential effects on the tissue are unclear. Here, the effect of these enzymes separately, as well as combined, was analyzed to determine their effects on morphology, protein content, and the proteome of the PCLS. The enzymes showed minimal effects on cell viability and protein content after two different incubation times, whereas morphological analysis confirmed collagen degradation by COL and COL(BEN), along with decreased ribosomal and secretory secretory protein levels. BEN decreased the levels of proteins related to protein synthesis and degradation. Increased levels of proteins related to detoxification and energy production were observed upon addition of COL, BEN, and COL(BEN). This study emphasizes the potential and risks for the use of collagenase and benzonase for EV isolation from the PCLS.

PMID 42918134
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PubMedJournal of agricultural and food chemistry2026-10-23

Recombinant Collagenase-Assisted Production of Bovine Bone-Derived Peptides and EGFR-Guided Discovery of Osteogenic Oligopeptides.

An Yanru Y, Wang Yu Y, Sun Yue Y, Su Dubilige D et al.

Bovine bone is a collagen-rich byproduct with potential for producing osteogenic peptides. A recombinant collagenase was heterologously expressed in Escherichia coliBL21(DE3), purified, and characterized. The enzyme showed a specific activity of 231.05 U/mg and hydrolyzed bovine bone collagen-containing matrix with a peptide yield of 58.12%. Nano-HPLC-MS/MS identified 1449 peptides in the most active fraction. EGFR-guided virtual screening prioritized GPYGPP and GPLGPA, with docking scores of -8.9 and -8.6 kcal/mol, respectively. At 250 μg/mL, GPYGPP and GPLGPA significantly increased MC3T3-E1 cell proliferation to 138.10% and 139.98% of the untreated control, respectively, and promoted cell-cycle progression, alkaline phosphatase activity, matrix mineralization, and osteogenic marker expression (p < 0.05). Transcriptomic analysis associated GPYGPP-induced osteogenesis with extracellular matrix remodeling and VEGF, HIF-1, and cAMP-related signaling. These findings provide an integrated strategy for producing bovine bone-derived peptides and identifying food-derived osteogenic oligopeptides with potential applications in bone-health functional foods.

PMID 42915780
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